１，lab

1, laboratory

laboratory

■ Instructor's room ■

Graduate school of Life and Environmental Science Research Building, (3F room No.303-1)

Intramural extension（7789）

TEL:Japan +81-25-262-7789（Message recording is possible）

FAX:Japan +81-25-262-7789

　The telephone passes only to office fundamentally.　 If you want to contact urgently, please access Dept. of Biology Office.
TEL : Japan +81-25-262-6267（to Mrs. Ikeda）

■Frog breeding room■

Graduate School of Life and Environmental Science Reserch Building, 3F room No.304-2

　The photographs of our frog room.　The J-strain Xenopus laevis to which we are maintaining the strain in this room is the only inbred strain of X. laevis in the world.　 This strain took J of Japan, was called J-strain, and has been established by Dr. Chiaki Katagiri and Dr. Shin Tochinai in Hokkaido University (Reference 1 and 2).　They have been established this J strain over 30 years.　The rejection for exchange transplantation among adults does not occur in the complete inbred strain.　However, in spite of the same strain, it was reported for the first time by our research that an adult rejected the skin of a larva (Reference 3).　J-strain of X. laevis is only in Hokkaido University and Niigata University in the world.　Most frogs are maintained in Niigata University now.
　At the frog room, We can do the thymus-extomized operation, the skin transplantation, and the micro-injection in the early stages of development.　At this laboratory, the poster of the contents of research is exhibited at any time.　We welcome those who interest to come to see.

Click the photos to magnify.

Reference

1、 Nakamura, T., Kawahara, H., and Katagiri, C. (1985). Rapid production of a histocompatible colony of Xenopus laevis by gynogenetic procedure. Zool. Sci. 2, 71-79.

2、 Tochinai, S., and Katagiri, C. (1975). Complete abrogation of immune response to skin allografts and rabbit erythrocytes in the early thymectomized Xenopus. Dev. Growth Diff. 17, 383-394.

3、 Izutsu, Y., and Yoshizato, K. (1993). Metamorphosis-dependent recognition of larval skin as non-self by inbred adult frogs (Xenopus laevis). J. Exp. Zool. 266, 163-167.

■ Experiment room ■

Graduate School of Life and Environmental Science Research Building, 3F room No.303-2

Tissue culture room

主に組織・細胞培養実験や，カエル胚・細胞への遺伝子導入実験を行っています。発生初期の胸腺摘出の手術や、皮膚移植も行っています。
生もの中心に扱っているので部屋の中はいつも温度が一定です。

Graduate School of Life and Environmental Science Research Building, 3F room No.304-1

Molecular room

PCRだの、大腸菌を増やすだの、いわゆる分子生物学の手法を用いた実験を行っています。実験台の上で寝たりしてません。実験しています。